In the present study, we chose the IHC method to evaluate cyclin D1 protein expression in HNSCC primarily because of the unavailability of fresh biopsy tissues. Although this method is a semiquantitative technique, IHC analysis is the most commonly used, simplest, and cheapest protocol in clinical work. Moreover, it is believed that cyclin D1 protein overexpression may occur via other mechanisms besides gene amplification, and the measurement of protein levels would be more informative than cyclin D1 DNA copies. According to the obtained results, low cyclin D1 expression at pretreatment forecasts a better clinical response and an improved DFS and OS in neoadjuvant chemotherapy patients. In this paper, a portion of sections were added P-CK stain to test the accuracy of CCND1 scoring area and to rule out the noncancerous cell stain. The results showed that the determined area and cells which were chosen by researcher and pathologists were specific and typical. The status of CCND1 expression was not changed after these sections reappraised according to the area and cells of the positive P-CK stain. Therefore, we have believed that the CCND1 stain alone combined with irregular AOI function of IPP 6.0 should be a reliable method of IHC scoring. And we have assumed that the method of score by IPP software is more objectivity than microscope count by observer. So the method may be the scoring trend of dyeing experiment such in future. In conclusion, our study indicates a key role of CCND1 in determining chemotherapy response and prognosis. We can select the patients with HNSCC who have the greatest chance of benefiting from neoadjuvant chemotherapy by CCND1 expression. Indeed CCND1 expression may serve as a predictive biomarker in selecting patients undergo future neoadjuvant chemotherapeutic clinical trials. Cholesterol is an integral component of the cell membrane and regulates the activity of ion channels in the lipid bilayer. Potential mechanisms of influence include: direct interaction with the channel protein, changes in the fluidity of the bilayer which affect ion channel gating and conformational change, or compartmentalization of ion channels into spatially restricted signaling complexes. The concept of lipid rafts emerged in the study of intestinal epithelial cells, where a polarized distribution of membrane lipids accompanies segregated INCB18424 trafficking at the apical and basolateral sides of the cell. Hair cells in the inner ear are uniquely asymmetric cells in both form and function, with a mechanotransduction complex composed of stereocilia embedded in a rigid cuticular plate located at the apical end and the intricate machinery of synaptic transmission clustered at the basolateral pole. While the finely tuned interplay of the ionic currents responsible for transducing the intensity, temporal, and frequency characteristics of sound to the brain is widely appreciated, the role of the local lipid environment in coordinating ion channel physiology in auditory hair cells is largely unexplored.