These results suggested that miRNAs may be involved in the bidirectional communication between oocytes and the regulation of amino acid metabolism in CRCs. Similarly, oocytes are also deficient in carrying out glycolysis and cholesterol biosynthesis. For instance, denuded mouse oocytes can undergo maturation in vitro by providing pyruvate in the medium, whereas oocytes co-cultured with cumulus cells mature in medium containing glucose as the only energy source. These results indicated that oocytes cannot use glucose directly and thus require cumulus cells to provide the pyruvate metabolised from glucose for energy consumption by oocytes. In consideration of the location of CRCs and COCs, the cumulus cells convert the glucose into pyruvate, which the oocyte can utilise via direct transport through the gap junctions of the CRCs or via secretion by COCs and subsequent membrane transport. In this study, the miRNAs were differentially expressed between CRCs and COCs, and after GO term annotation and pathway analysis we suggest that the energy substances supporting oocyte development and maturation might be primarily obtained from the production of CRCs under the regulation of miRNAs. Oocytes seem to lack the complete enzymatic system required for the synthesis of cholesterol, such as Mvk, Pmvk, Cyp51, Fbps, Sqle, and Ebp. In addition, the cholesterol receptors, e.g., SCARb1 and LDLR, are also not expressed in mouse oocytes. Furthermore, several studies also indicated that cholesterol from cumulus cells is the main source of oocyte cholesterol. Our data suggested that miRNAs in the CRCs might be involved in cholesterol biosynthesis and the transport of cholesterol into the oocytes. In conclusion, oocytes undergo a prolonged and carefully regulated developmental process as a result of junctional interactions and instructive paracrine signalling with CRCs and COCs. The miRNAs seem to play a key role in the exchange of nutritional materials and regulatory signals between the oocytes and surrounding cumulus cells. The immune system seems to regulate the development of the follicle and the corpus luteum, and its maintenance and regression, via the ovarian granulosa cells. Meanwhile, NCOR1 is also a component of the tamoxifen/oestrogen and receptor tyrosine kinase signalling pathway. Furthermore, ovulation was found to be associated with tissue remodelling and inflammatory molecules at the site. These findings suggested that miRNA-induced immunity regulation, such as the regulation of T cell biology, perhaps participates in ovarian cumulus cell-related processes. In summary, for the first time we have analysed known and novel miRNAs in human stimulated Tubulin Acetylation Inducer preovulatory luteinizing CRCs and COCs by high-throughput Solexa sequencing. We have detected similarities and differences in the miRNA expression profile between CRCs and COCs, and confirmed their expression by quantitative real-time PCR analysis.