Further modifications were made to fatty acid oxidation. The fatty acid used by Ramakrishna, palmitate, is a 16 carbon fatty acid. Although the same enzymes are used in each cycle of the fatty acid oxidation, each cycle here is detailed. In other words, there are reactions which oxidize a 14 carbon fatty acid, a 12 carbon fatty acid, etc. This makes it possible to add other fatty acids and to incorporate amino acid oxidation into the same cycle. During certain conditions, the muscle will supplement or convert to amino acid oxidation for energy supply. The significance of these results is apparent when comparing the amount of amino acids needed to build one contractile protein complex to what is available. One would expect that AbMole 3,4,5-Trimethoxyphenylacetic acid methionine and isoleucine, respectively, should be the top two amino acids needed to increase protein flux because these essential amino acids have the largest differences between what is available and what is needed. As expected, this is also what the model shows. However, it is interesting the model then predicts that leucine and valine are the next important essential amino acids necessary at increasing the protein flux even though, based on availability differences, one would expect phenylalanine and tryptophan would be the next effective supplements. Arginine, a conditionally essential amino acid is also greater than expected in supplementation affect based on essentiality and availability differences. We obtain similar results when investigating the results in post-absorptive state. According to the rank between what is available and what is needed, methionine, isoleucine, and leucine should be the most effective, but in fact the model shows that phenylalanine and arginine are more effective. The advantage of using this metabolic modeling is being able to track the fluxes for each of the metabolites. Thus, we can predict why our results differ than what is expected if availability is the only factor. Not only is there an increase in contractile protein synthesis when relaxing the constraint on methionine, the model predicts that there is a change in how energy is produced in support for protein synthesis. As seen in Figure S1E, there is a decrease in glycolysis from glucose and a decrease in fatty acid oxidation from palmitate. Some of the energy for the synthesis process comes from increased lysine oxidation among changes in other amino acid catabolism, such as asparagine, serine, and glycine. Glutamine is predicted 
to be made in excess and transported out of the muscle tissue. This process increases the TCA cycle and the NADH shuttle to the mitochondria. Although relaxing the constraints on the other single amino acid supplementation conditions did not increase contractile protein synthesis, it did alter the metabolic profile of the network compared to the control condition. Here we will describe a few of the different profiles based on different supplementation conditions.