The subject’s reactive aggression was assessed using the number of points he subtracted from the opponent on a winning trial following a losing trial, with a certain level of provocation. The game lasted 48 trials with a 1-minute break after 24 trials. Before the game started, the participants were asked if they were nauseous and if they agreed to complete a short in-house questionnaire on how they felt before and after the PSAG. However, the global distribution of the parasite has now extended perhaps as a result of unintended importation of definitive rodent hosts on ships and aeroplanes. Recently, the Canary Islands have been added to the distribution area of this nematode. The life cycle of A. cantonensis involves rats and mollusks as definitive and intermediate hosts, respectively. Humans are accidentally infected through the consumption of raw or undercooked mollusks that contain the infective third stage larvae. Infection can also take place by eating animals that act as a paratenic host or ingesting contaminated fresh vegetables, including raw vegetable juice. After ingestion, the nematodes are digested from tissues and enter the bloodstream in the intestine. The larvae can finally reach the central nervous system or the eye chamber, causing eosinophilic meningitis or ocular angiostrongyliasis, respectively. During the past decades, several outbreaks of human angiostrongyliasis caused by A. cantonensis have been documented worldwide. Since 1945, more than 2800 cases of human angiostrongyliasis by A. cantonensis have been reported in approximately 30 countries. Nowadays, this zoonosis is considered an emerging tropical disease. Human cerebral angiostrongyliasis presents a broad Diatrizoic acid clinical spectrum, from a mild disease to a form of eosinophilic meningitis or, uncommonly, encephalitis. As a result, neurologic damage and even death may develop, especially if prompt and proper treatment is not administered. The suspected diagnosis can only be confirmed upon finding and identification of A. cantonensis worms from the cerebrospinal fluid of infected patients, but this rarely occurs. Consequently, over the past decades a great number of immunological tests have been developed to enable the diagnosis of this human angiostrongyliasis. These approaches include an Indirect Enzyme Linked Immunosorbent Assay using a 31-kDa glycoprotein from the adult worm. This glycoprotein is among the principal Nortriptyline antigens recognized by sera of human with A. cantonensis as well as sera of immunized mice, rats and rabbits. Previous studies in human have shown 100% diagnostic sensitivity and specificity on testing sera by ELISA, when 31-kDa glycoprotein is purified through electroelution from SDS-polyacrylamide gel. The high density of rats on the Canary Islands and their role in the life cycle of A. cantonensis highlighted the need of an epidemiological study in this archipelago.