BHK cells Mepiroxol contain 4 major GPI-anchored proteins, N-CAM-140, semaphorin-7, CD14 and Thy-1, which can be detected by overlay using the GPI-specific bacterial toxin aerolysin. Our finding that such raft-like membranes, containing GPI-anchored proteins, are present within intralumenal membranes of these multivesicular endosomes fits Ginkgolide-A nicely with electron microscopy observations using a cholesterol-binding toxin showing that cholesterol is abundant within these lumenal membranes. It has recently been shown that GPI-anchored proteins can be endocytosed from the plasma membrane via a flotillin-1 dependent pathway. Understanding how GPIanchored proteins and flotillin-1 segregate from one another at later stages of the endocytic pathway will be of great interest. Importantly, both limiting and luminal membranes also contain fluid membranes as illustrated by the detergent sensitivity of lamp1 and LBPA respectively. A total of 122 probes had significant hybridisation differences in GM and conventional samples. These values are similar to those obtained by mRNA-seq and also to those reported in MON810 versus nearisogenic leaf tissue and mature grains grown in agricultural fields. Although the general numbers of regulated sequences are similar by using mRNA-seq and microarray hybridization, just 82 sequences were identified through the two technical approaches. Fifty-eight additional sequences were only identified by mRNAseq and 35 were uniquely detected using microarray hybridization. This reflects the differences inherent in each technology. Similarly all 58 gene transcripts showing differential expressed in GM and conventional embryos as determined by mRNA-seq were not included or annotated in the microarray probeset, thus they were not investigated in the microarray hybridization experiment. It is differentiated from its wild progenitor principally by thick glabrous stem and erect growth habit and reported to be cultivated as pulse in Vietnam and the Philippines or as forage in India, Mauritius and Tanzania. This crop shows resistance to several insect pests and diseases such as bruchids, bean fly, powdery mildew, and cucumber mosaic virus, and is partially cross-compatible with mungbean.