Together with Cytoscape it enabled the visualization of the distribution of the biological processes among the identified proteins, the number, identity and type of each protein in each process, the relative fold change levels of each protein and their interactions,1-Deacetylnimbolinin-B all resultant data integrated in the same network. It was also possible to analyze the network according to the enriched KEGG pathways and GO cellular components, since these information were also computed and available in the generated network. In a more global perspective, it was of immediate observation that the majority of up-regulated proteins was involved in cellular metabolic processes, unlike the downregulated proteins, mostly involved in RNA processing, translation and cellular component organization or biogenesis. Regarding the metabolic process clusters in the network, as also emphasized by Westman et al., the glycolytic pathway enzyme Tdh1p was found in a significantly higher level in the encapsulated yeast, and the high affinity hexose transporters Hxt6p and Hxt7p, although not clustered together, were visually identified as the most up-regulated proteins. Moreover, our analysis was able to identify many proteins in the glycogen biosynthetic process cluster, and proteins involved in NADH oxidation, which were all up-regulated. These findings strongly indicate a carbon limitation inside the capsules, but an accumulation of glycogen as the capsules filled up with cells,1-Tigloyltrichilinin considering its importance as a storage carbohydrate in slowly growing or starved yeast, and, more relevant, an increase in ethanol yields. Notably, proteins involved in the ergosterol biosynthetic process cluster were also visually identified as greatly up-regulated, although not discussed in the previous report by Westman et al.. Since ergosterol is the major sterol of the fungal plasma membrane, important for the fluidity and integrity of the membrane and for the proper function of many membranebound enzymes, with its biosynthetic pathway consisting in a pivotal target of antifungal drugs, these findings may also explain the differences between encapsulated and free growing yeast cells. Indeed, a more intact membrane supports higher concentrations of ethanol.