This analysis adds novel possible clues on how hNek6 activates NF-kappaB cascade. Although the Transcription factor RelB was found to interact only with hNek6 from the referred cluster, it is already a direct link to the NF-kappaB cascade activation, since it is a component of the NF-kappa-B RelB-p50/p52 complex. Nek6 is also directly linked to Proteintyrosine phosphatase-like A domain-containing protein 1,Salannal enriched in the I-kappaB kinase/NF-kappaB cascade cluster. Altogether, these findings may suggest a novel non-mitotic function for hNek6 through this pathway. As an example of a proteomics study, we chose the S. cerevisiae proteome of encapsulated cells in liquid core alginate-chitosan capsules in comparison with cells grown freely in suspension described by Westman et al.. In the context of bioethanol production, encapsulation of yeast cells has been shown to improve the fermentative performance in toxic lignocellulosic hydrolysates and to increase thermotolerance. It has been shown that the yeast metabolism changed significantly upon encapsulation, so we used IIS to build a network for the 116 up- and 95 down-regulated proteins in yeasts growing in capsules to comparatively analyze how encapsulation affects the cells on a more integrated molecular level. First, we uploaded a single two-column TXT file containing both the up- and down-regulated proteins, available as UniProt Accession numbers and respective fold change values, in the ‘‘Module 2: Search’’ tab inside the project. Then the retrieved proteins were selected and added to the project, annotated in the ‘‘Module 3: Annotation’’ tab,12-Ethoxynimbolinin-B and used as queries to build a network in the ‘‘Module 4: Interactome’’ tab, setting expression analysis parameters to consider fold change $1.3 as up-regulated and fold change #21.3 as down-regulated proteins. The network was visualized and manipulated using the Cytoscape software. Figure 4 shows the interactome of encapsulated S. cerevisiae built from the proteome data. Our new analysis using IIS showed the same and other functional categories enriched among the up- and down-regulated proteins as described before, but using the GO database instead and with one considerable advantage.