Most of the differentially expressed genes in microglial following LPS stimulation were expressed as several isoforms subjected to transcriptional/post-transcriptional regulation and/or differential promoter usage. We Procaine hydrochloride classified these genes into three main groups. The first two Ropivacaine hydrochloride groups included genes crucial for the innate immune response, which might be under stronger selection to prevent the emergence of new isoforms and/or post-transcriptional regulation. In addition few of the genes belonged to the third group, suggesting that they could be subjected to positive selection at the transcriptional and post-transcriptional regulation in LPS stimulated BV-2 microglial cells. However, further targeted studies are required to validate this regulation and establish the potential effects of these genes during microglial infection. Epigenetic regulation, which involves chemical modification of DNA cytosine residues and DNA-bound histone proteins without alterations in the DNA sequence, is promising as one of the major factors regulating gene expression in response to environmental stimuli. Recent studies have demonstrated that histone demethylases and histone deacetylases potentially regulate proinflammatory gene expression in macrophages. Recently, we showed that the histone demethylase kdm4a was significantly expressed in neuro ectodermal stem cells and might play a role in tumorigenic development. Interestingly herein, the RNA-Seq data also revealed that the histone demethylase Kdm4a and DNA methyl transfer as eDnmt3l were strikingly differentially expressed in LPS stimulated BV-2 microglial cells. However, the histone demethylase Kdm6b and histone deacetylases, hdac1, hdac2, hdac3, and hdac7, were not affected in LPS-stimulated BV-2 microglial cells. The top KEGG pathways identified in DAVID included immune system processes and stimuli responses, while the top canonical pathways identified in IPA involved the communication between innate and adaptive immune cells and pattern recognition receptors in recognition bacteria and viruses.