Among the proteins identified in mesogel, Hsp47, Tnfrsf11b, Anxa2, Myh10 and Myh11 were observed to promote AT7519 hydrochloride differentiation of MSCs into osteogenic, chondrogenic or myogenic lineages while Ina and Lpl were found to be markers of differentiation into neuronal and adipogenic lineages. The absence of these proteins in cardiogel could have contributed to the enhanced cardiomyogenic differentiation and decreased osteogenesis that was observed in cardiogel. Few proteins like Gsn, Vdac2 and Hspa1l were known to be involved in general cellular component organization such as assembly and disassembly of actin filaments, regulation of mitochondrial apoptosis, protein stabilization against aggregation and protein folding respectively. Biomaterials to be used as scaffolds for cardiac tissue construct, should be biocompatible, and should exhibit functional and morphological properties of native heart. Cytocompatibility studies proved that cardiogel increased cell proliferation, adhesion and migration. These effects could be attributed to the synergistic effect of proteins such as Tmsb4x and Psap, which were known to improve cell survival and migration and also prevent apoptosis of cardiomyocytes under stress. Cardiogel also promoted cardiomyogenic differentiation of BMSCs. Previous studies have Oxytetracycline reported that 5-azaC treatment induces BMSCs to elongate and develop into myotubule-like multinucleated structures. However, similar morphological changes were observed in BMSCs cultured on cardiogel, even in the absence of any chemical induction. RT-PCR analysis showed an increased expression of cardiac transcription factors such as Gata4 and Mef2c, the major cardiac gap junction protein Cx43, a cardiac hormone BNP, the adrenergic receptor Adra1a and Adra1b and the muscarinic acetylcholine receptor, Chrm1 while Western Blotting showed an increase in the expression of GATA4, ACTA1 and CX43 in BMSCs cultured on cardiogel compared to those cultured on gelatin coated controls. The cardiomyogenic differentiation of BMSCs on cardiogel could be contributed to the presence of proteins such as Tmsb4x, A2m, laminin and fibronectin, which are known to enhance cardiomyogenic differentiation of stem cells.