The potential of using these deconstructed biomass materials as a carbon source for fermentation not only overcomes the expense associated with using a chemically more complex, recalcitrant carbon source, but also bypasses the high cost and poor yields of enzymatic hydrolysis of waste biomass into simple sugars that are suitable for fermentation. Rhodosprillum rubrum is an attractive microbial fermentation organism for converting such deconstructed biomass-products to value-added Morphine sulfate salt pentahydrate biochemicals because it can utilize a variety of different carbon and energy source under anaerobic conditions. Because of its flexible capabilities to grow aerobically, anaerobically or as an autotroph, R. rubrum is particularly attractive for fermenting syngas feedstocks, which are primarily a mixture of carbon monoxide, hydrogen, carbon dioxide and methane. In this study we explored the potential of using this Gram negative, photosynthetic purple non-sulfur bacterium for the production of valuable biochemicals from simple carbon feedstocks, using a transcription regulatory system that is inducible with carbon monoxide, which would therefore be applicable in developing a syngas fermentation platform. The biochemicals we targeted for these bioengineering efforts are polyhydroxyalkanoates, which are biodegradable polyester polymers that are deposited within inclusion bodies, and many microbes use them as a means of storing carbon and energy. Because PHAs can be used as biodegradable plastics there has been a great deal of interest in generating a PHA production system based upon microbial fermentation. R. rubrum has the potential of accumulating up to 50% of dry weight as PHA, using an optimal carbon source such as butyrate. Recent studies have evaluated the technical and economic feasibility of using such a microbial platform for the conversion of biomass feedstocks to biofuels or biochemicals, and one of the conclusions from these studies is that additional ����metabolic engineering could be employed to increase yield and broaden the variety of available products����. In this study we specifically targeted the bioengineering of R. rubrum PHA biosynthetic genes, and explored the effect of systematically overexpressing them on the production of PHA, when R. rubrum is grown in chemically simple carbon feedstocks in anaerobic Naltrindole isothiocyanate hydrochloride conditions, and controlling the expression of the bioengineered genes with a carbon monoxide inducible promoter.