Artemisinin are present in various plant organs, including leaves, stems, floral and fruit parts. However, there are large differences found in artemisinin content, depending on the variety, season, cultivation condition, and plant developmental stages. Previously, it was shown that artemisinin has potential to inhibit the seed germination of different plant species ; although no detailed research has been conducted regarding artemisinin impact on the growth of surrounding plants, or physiological and biochemical metabolism. Lydon et al. found that the incorporation of Abn-CBD Artemisia annua dried leaves in the soil provided good weed control, but the level of phytotoxic activity was independent of the concentrations of artemisinin in soil, suggesting that other factors may play important roles. Secondary plant metabolites play a variety of physiological roles and have a number of advantages over the synthetic herbicides as they usually have structural diversity and have novel target sites of action. Despite the pharmaceutical importance of plant-derived artemisinin, relatively little is known about the biochemical, physiological and isotopic responses to artemisinin in target plants. Here, we studied the fundamental biological processes by investigating the Arabidopsis plants�� dynamic response to phytochemical treatments, interference in growth, development and physio- biochemical characteristics. In accordance with DiTomaso & Duke and Lommen et al., we confirmed that artemisinin was able to decrease the fresh biomass in a dose-dependent fashion. Chen and Polatnick reported that the fresh weight of mung bean seedlings treated with artemisinin was 19�C26% less as compared to control. In this experiment, the reduction in the carbon and nitrogen content of Arabidopsis leaf was observed after treatment with artemisinin at 160 ��Mconcentration. Lydon et al. found that Artemisia annua leaf tissue incorporated into the soil decreased the soybean biomass in a pot culture experiment. In this study, artemisinin increased the 2-MPMDQ sodium, potassium, phosphorus, aluminium and copper contents. Conversely, S��nchez-Moreiras et al. found a reduction in leaf nutrient contents in Arabidopsis after treatment with BOA. In all photosynthetic organisms, chlorophyll plays a central role in the harvesting and photochemical transformation of light energy into chemical energy. The excitation energy absorbed by the light harvesting complex can usually undergo three fates; it can drive photosynthesis, be dissipated as heat or as red fluorescence. These three processes occur alongside each other. Therefore, determining the yield of chlorophyll fluorescence will give information about changes in the efficiency of photochemistry and heat dissipation. Damage to cell membrane indicated by higher concentrations of MDA content that was observed in artemisinin induced stressed Arabidopsis plants when compared with untreated control.