Here we show that the majority of plasma protein changes identified in these tumor models were unique to each model and not seen in the confounder models. Furthermore, many of these proteins have known roles in cancer progression. The identification and characterization of protein profiles associated with cancer versus non-cancerous pathologies can be used to understand the complex biology of the host response to cancer and to prioritize candidate biomarkers that are associated with cancer. Animal studies were performed under IACUC regulations as approved by the FHCRC animal use committee. Ten female FVB mice were used for each condition paired with ten littermate untreated controls. To model acute inflammation that transitions into subacute inflammation, we used a well known pro-inflammatory irritant, carrageenan, a carbohydrate derived from seaweed. This was delivered via a sponge implant which sustains the carrageenan release and contributes a classical foreign body response. 10610 mm Fingolimod customer reviews surgical sponges were AZ 960 injected with 1% carrageenan and implanted subcutaneously into the right flank. Plasma was collected by cardiac puncture 3 weeks later. The plasma proteins identified from these mice should correspond to a late stage subacute response to the carrageenan and associated sponge impact, rather than to initiation of the acute inflammatory response which occurs within 72 hours. To evaluate the protein profile associated with chronic inflammation, we used a collagen-induced arthritis mouse model. Bovine collagen type II was emulsified with complete Freund��s adjuvant at a final concentration of 4 mg/ml and a total of 0.1 ml was injected intradermally near the base of the tail. This results in the development of chronic arthritis in the hind paws within 14�C21 days. Mice were monitored every 2�C3 days for the development and progression of arthritis and plasma collected upon development of swollen hind paws at 4 weeks. To model angiogenesis, matrigel plus FGF was injected subcutaneously into the right flank resulting in rapid in-growth of blood vessels and supporting stromal elements but with little associated inflammation. Plasma was collected 3 weeks later. For these models, blood from experimental and control mice was collected by cardiac puncture, using a 1 cm3 syringe and a 23 g needle, and placed in a K3-EDTA tube. Plasma was isolated by centrifugation at 20006g for 5 min and aliquots transferred to cryovials and frozen at 280uC. Sample collection for the pancreatic and breast cancer mouse models has been previously described. This difference in euthanasia method introduces a potential, although likely minor, caveat when comparing the pancreas models to the other models. A 1-mL syringe with a 22 g needle was used for cardiac puncture to obtain blood. Blood was placed in K3-EDTA tubes and centrifuged at 4uC for 5 min at 3000 rpm.