Looking at the technical controls in GenomeStudio, one of the samples had a different distribution of signals in several control plots, such as the box plot visualising the variation within an array and between arrays, the line plot of detected genes, the line plot of noise and the line plot of labelling control across samples. All samples were included in further quality control, outlier detection, and pre-processed using the J-Express software version 2009 . Quality control and analysis in this software was done on log2-transposed data. Correspondence Analysis and hierarchical BYL719 biological activity clustering with Pearson Correlation as a distance measure were used on both the un-normalised and quantile normalised dataset . The Correspondence Analysis plot was used to look for greatest co-variance in the dataset. Sample C-111 was an outlier in the un-normalised dataset in the Correspondence Analysis plot, and together with the outlier detection of control probes in GenomeStudio we decided to exclude this sample before further analysis. Significance Analysis of Silmitasertib Microarrays was used to look for differentially expressed genes, which were defined by qvalue, 0.05. Protein Analysis Through Evolutionary Relationships was used to organise differentially expressed genes in categories representing biological processes and molecular functions. We looked for overrepresentation of differentially expressed genes in such categories, relative to the expected representation in the whole genome. The Bonferroni correction for multiple testing was used in the calculation of p-values for the over-represented PANTHER categories. Patients with PHPT are at increased risk of CVD, which may be due to a chronic low-grade inflammation. In the present study we investigated the gene expression profile of PHPT patients in subcutaneous adipose tissue from the neck. Our results indicate that patients with PHPT have inflammatory and metabolic changes in their adipose tissue. Pro-inflammatory stimuli alter the expression of adhesion molecules on the endothelium, mediating endothelial attachment of circulating lymphocytes and monocytes and initiating early steps of atherosclerotic lesions . It has previously been shown that the subcutaneous adipose tissue in morbidly obese bariatric patients expresses high levels of inflammatory genes, particularly in stromal vascular cells .