These enzymes catalyze the 3rd, 4th,5 th, and 8th steps of the plastidic porphyrin biosynthesis, respectively, providing the backbones for StemRegenin 1 chlorophyll and heme molecules. The identification of porphobilinogen synthase and glutamate 1-semialdehyde aminotransferase as targets of Snitrosylation is described for the first time. Taken together, the identification of enzymes involved in glycolysis, the pentose phosphate pathway and chlorophyll biosynthesis as targets of Snitrosylation in plants raises the general question as to how Snitrosylation is globally involved in the fine tuning of the photosynthetic activity, pigment turnover, Calvin cycle processes and subsequent channeling of the photosynthetic metabolites that are related to the modifications of the glycolytic and pentose phosphate pathway proteins. To provide information regarding the biological relevance of Snitrosylation, the comparative analysis of the S-nitroso-proteome under control and stress conditions is an important tool. At the present, no information is available regarding ozone-induced changes in the S-nitroso-proteome of plants and this is the first study that comprehensively describes it. Here, we applied a short, strong ozone pulse as a model to trigger ROS and NO formation in order to analyze the fast responses in denitrosylation. Short-term acute ozone fumigation is often used to mimic the HR and therefore the present shifts in the S-nitrosylation Ampicillin sodium pattern might be transferable to early events in leaf pathogenesis. The accumulation of NO and nitrite is a common feature of short term and chronic ozone fumigation. We observed a rapid nitrite increase and a slight increase in the nitrosothiol content in response to the short-term ozone treatment. Increased nitrite content is often observed upon abiotic stresses and is linked to S-nitrosylation events. It has been shown that nitrite induces S-nitrosylation and the subsequent inactivation of the protease caspase-3. Nitrite, a reservoir for NO, can be reduced back to NO via non-enzyme-dependent reactions or enzymatically by nitrate reductase. Higher levels of SNOs comprise GSNO, a low-molecular weight SNO.