Early-stage RMS patients are often confused with EWS, consequently inducing lymphaticrelated cancer when the RMS tumor migrates to lymph node. Although these tumors have similar clinical conditions, EWS is commonly developed in bones, whereas RMS is more frequently found in Tenatoprazole skeletal muscle. CSDA is a repressor gene involved in various biological processes including skeletal muscle tissue development and organ growth. FHL3 is only expressed in skeletal muscle and could be involved in tumor suppression and repression of MyoD expression. IFITM3 is IFN-induced antiviral protein that plays a role in innate immune response to virus infections. NFIC is a cellular transcription factor involved in DNA binding transcription factor activity. Although the function of TRIP6 is not fully understood, it has been associated with ligand binding of the thyroid receptor in the presence of thyroid hormone. TAF15 plays specific roles during transcription initiation in RNA binding and it may be involved in protein-protein-interaction. RXRG is a retinoic acid receptor that regulates gene expression in various biological processes, including skeletal muscle tissue development, heart development, and response to hormone stimulus. RXRG is a tumor suppressor gene that mediates the antiproliferative effect of retinoic acid, an essential metabolite of vitamin A for the growth, development and cell differentiation of vertebrate species. This protein suppresses tumor growth by increasing the anti-proliferative effects of RA in the tumor cells. MYL1 is the motor protein known for the role in muscle cell activities including vesicle transportation inside the muscle cell. A negative interaction on this protein indicates that tumor cells are Proflavine Hemisulfate constrained in a particular location rather than move randomly. FHL3 expressed only in skeletal muscle has been known by its role in skeletal myogenesis, although its actual function is unknown. This gene has been related to cell spreading and actin stress fiber disassembly and is involved in tumor suppression/ repression of MyoD expression. RND3 is a member of the Rho family GTPase protein superfamily that acts as a negative regulator in cytoskeletal organization. It is known to have a role in myoblast fusion and to be responsible for down-regulation in focal adhesions and stress fibers.