Moreover, the mini-V3 in a non-scaffolded context failed to be recognized by broadly neutralizing antibodies, indicating that the structural integrity provided by the scaffold was critical to the glycan V3-supersite transplants. Only a portion of the glycan V3 supersite was transplanted, and the degree of antigenic mimicry against antibodies from a specific donor correlated with Cycloastragenol the portion of the transplanted supersite recognized by antibodies from that specific donor. A design that incorporates the transplantation of a larger portion of the supersite may have greater antigenic breadth. In this regard, it should be noted that the glycan V3-supersite is one of the most promiscuously recognized of the HIV-1 Env supersites of vulnerability. Antibodies that recognize this supersite range from PGT122, which binds a glycopeptide V1-V3 spanning epitope,Calycosin-7-glucoside to PGT135, which binds a glycopeptide V3-V4 spanning epitope, and to 2G12, which binds a glycan-only epitope. It remains to be seen whether transplants comprising greater portions of the glycan V3 supersite would make more effective probes or immunogens; we note in this context that it remains to be shown whether the glycan V3-supersite transplants described here can elicit effective HIV-1-neutralizing antibodies – either alone, as cocktails, or as components in prime/boost regimens. Nevertheless, the structural and antigenic analyses described here, along with the successful oligomerization of the glycan V3supersite transplants in the ferritin nanoparticle context, do demonstrate that supersite transplants with antigenic mimicry of the template supersite can be achieved through transplantation of only a portion of the recognized supersite. Overall, the design framework and described glycan V3-supersite transplants provide both conceptual context and initial immunogens for a supersitefocused vaccine effort. The disease is known to cause progressive fatal disease in poor people particularly belonging to North-eastern part of Indian subcontinent. An average of more than 90% of VL cases in India is reported from Bihar alone. Recent epidemics of VL in Sudan and India have resulted in over 100,000 deaths.