Among these 36 miRNAs, 15 miRNAs corresponded to 447 target genes were of general consensus across the three databases and selected for further study. Expression regulations of 8 miRNAs among these 15 miRNAs were Forsythoside-A validated by real time PCR: hsa-miR-409-3p, hsamiR-487b, hsa-miR-155-5p, 6-gingerol hsa-miR-379-5p, hsa-miR-222-3p, hsa-let-7e-5p and hsa-miR-433 were downregulated, while hasmiR-498 was upregulated, showing the two methods gave consistent results. Among target genes of these 8 miRNAs, some were associated with cardiac development. Further functional significance analysis of these target genes showed that those associated with the most enriched GO functions regulated development of right ventricular functional morphology. Key target genes included NOTCH1, HAND1, ZFPM2 and GATA3, which were regulated by three miRNAs of the eight miRNAs validated by RT-PCR: hsa-let-7e-5p, hsa-miR-222-3p and hsamiR-433. The expression of the three miRNAs were significantly downregulated in the plasma of patients with VSD. The results presented here suggested that hsa-miR-433 may regulate target genes NOTCH1 and GATA3. Previous studies have shown that NOTCH1 is closely related with ventricular development. During embryonic development, NOTCH1 is expressed in the endocardium at the bottom of the trabecular layer. NOTCH1 knockout in rats causes trabecular defects, and NOTCH1 expression is downregulated in fetal myocardial tissue with VSD. The GATA family has six genes. GATA4 is a transcription factor that plays a regulatory role in a heart-mediated regulatory network, and is involved in cardiac development and expression of functional genes. The GATA4 gene plays a key role in compartmentalization of the heart, atrial and ventricular development, atrioventricular valve formation, and arterial trunk separation, and it is essential for the heart to develop, mature, and play its physiological functions. GATA4 may also be important for repair and reconstruction post-myocardial damage. GATA3 is specifically expressed in T helper 2 cells, and is important for Th2 cell proliferation, differentiation and development.