It is also supported by data found in public databases, which showed that C/EBPb and galectin-7 have an almost identical distribution pattern in both normal and cancer tissues. Taken together, these results identify a novel regulatory pathway that regulates galectin-7 expression in human breast cancer cells. Gene expression analysis is becoming more important in many biological fields, such as applied functional genomic research and the study of infection processes. Consequently the quantification of gene expression has to be accurate. Due to its high sequence-specificity and its tremendous sensibility, quantitative real-time RT-PCR has become the method of choice for quantifying the expression of selected genes in an increasing number of biological samples. However, noisy technical variations, such as RNA extraction efficiency, RNA quality or cDNA synthesis efficiency, in different biological samples could interfere with the final expression measurements. Consequently, data generated with RT-qPCR should be normalized to compensate for these variations. The most Nalidixic acid sodium salt frequently used method to minimize such variations is relative normalization, where the expression of a target gene is quantified with respect to stably expressed internal reference genes. Indeed, recent studies Mastoparan-7 demonstrated that only the use of several reference genes results in accurate normalization. Genes used for normalization should be expressed stably in the conditions of interest. Housekeeping genes have been commonly used as reference genes. Indeed, these genes are described as being essential and ubiquitous. Unfortunately, housekeeping genes have recently been demonstrated to be highly variable under several experimental conditions. The use of such inappropriate reference genes in the relative quantification of gene expression could result in biased expression profiles. Therefore, finding stable reference genes is becoming an essential prerequisite for a reliable measurement of gene expression. Dickeya dadantii is described as a macerogenic Gram-negative plant pathogen that causes disease in a wide range of plant species, including many crops of economic importance such as vegetables, ornamentals and also the model plant Arabidopsis thaliana.