The use of plant-produced signals, such as the damage-induced Evofosfamide release of pregeijerene, along with conservation biological control strategies, could extend the usefulness of EPNs in crops damaged by belowground herbivores. The obstacles of investigating belowground chemically mediated interactions between plants and animals are being overcome gradually, opening opportunities for manipulating these interactions for enhanced biological control. At least half of all plant biomass is attacked by underground herbivores and pathogens, living in a complex ecological food web in the soil. Although induced plant responses were originally postulated as a potential novel approach to pest management in agricultural systems for insect herbivore population regulation, few studies of induced responses have addressed their practical application beyond fundamental concepts in ecology and evolutionary biology, with particularly few studies for belowground systems. HIPVs are likely important mediators of tritrophic interactions that afford indirect plant defense within the root zone. Our study not only shows this approach in the field, but also provides the first description of an ecological role for the C12 terpene, pregeijerene. To evaluate applied volatiles for the attraction of belowground natural enemies in the field, studies usually quantify mortality of a target pest by trapping adults emerging from soil. This technique frequently results in low recovery and also gives no confirmation of the specific cause of mortality. In addition, it can be difficult to quantify populations of naturally occurring EPNs, which may be abundant in soil, but remain cryptic. We used realtime qPCR as an efficient method for describing EPN diversity and quantifying their abundance. Moreover, we showed that pregeijerene was directly responsible for attracting five species of native EPNs in the soil so as to enhance pest mortality. Given the efficacy of this compound, there may be little need for exogenous application of non-native EPNs in systems with a rich fauna of native EPNs. In orchards with established EPN populations, large-scale introduction of non-native species may temporarily reduce native populations due to trophic cascades that increase predatory fungi and attenuate net efficacy of biological control. Although it is known that artificially reared and commercially formulated EPNs can persist, it is possible that natives have advantages associated with habitat acclimation and response to HIPVs; thus, further investigation of enhancing conservation biological control of belowground pests in concert with behavioral modification via HIPVs is warranted. The results of the experiment conducted in blueberries, an agricultural setting vastly different from citrus, demonstrate the potential broad applicability of pregeijerene on diverse species of EPNs. Timing application of pregeijerene to target the most susceptible instar of A. orientalis should optimize its efficacy -instar A. orientalis may be less susceptible to EPN infection than earlier larval instars.