For instance, females rest or feed, whereas males are highly agitated, even when they enter an aphid colony. This behavior might be interpreted as mate-searching behavior, and could be associated to the production of a volatile sex pheromone by females. In this work, we set out to record whether virgin female lady beetles exhibit the typical “calling behavior” that has been previously associated with the emission of a sex pheromone in several other Coleoptera species. Subsequently, volatile collection was performed to identify and quantify the chemical components of the emitted blend, and the behavioral response of conspecifics was tested. The findings of this study are expected to expand knowledge about sex pheromone production in the Coccinellidae family, and would contribute towards improving biological control methods involving H. axyridis whereas males did not respond to this cue. The mismatch between the present study and previous research might be explained by the different concentrations of -b-caryophyllene that were tested; specifically, the pure compound from chemical synthesis, chemical formulation in paraffin oil, or, in this case, as a component of a natural sex pheromone. Indeed, insect behavioral responses are known to vary according to the concentration of the volatile being applied. Furthermore, an electroantennographic bioassay highlighted the existence of neuronal receptors allowing the perception of -bcaryophyllene in this species, and showed that male antennae were more sensitive to this semiochemical compared to female antennae. When analyzing the emission profile, we highlighted that virgin females start to emit volatile cues 3 days after being fed A. pisum. The chemical analyses revealed that all of the compounds exhibited a similar emission profile, whereby their quantities gradually increased across the sampling period. This observation strongly supports that -b-caryophyllene, b-elemene, methyl-eugenol, Afatinib ahumulene, and a-bulnesene are part of the pheromonal blend. This work provides the first evidence confirming that sexually receptive females exhibit a characteristic behavior. This behavior contains important similarities to what has been previously termed “calling behavior”, which has been described for several Coleoptera species in the Cerambicydae family and the Dermestidae family, in addition to cockroaches. The female calling behavior of many insect species is usually associated with the release of a volatile sex pheromone. The synchronized production of chemicals and behavioral responses was also obtained for the multicolored Asian lady beetle in the current study. The calling behavior was observed 3 days after the lady beetles were fed aphids, which directly coincides with the onset of pheromone emission.