Moreover, this study provides an important reference for the development of novel therapeutics on promoting blood circulation that employ angoroside C, calycosin-7-O-b-D-glucoside, panaxytriol, and protocatechualdehyde. Besides, according to the attribution of 6 bioactive components, PN might affect two aspects of the vascular system, including F1 and F4, while RA, RS, and SM might influence the F5, F2, and F3, respectively. Consequently, this work also partly revealed the mutual promotion among 4 herbs and demonstrated the multicomponent and multitarget properties of CXC. However, the results also showed that rosmarinic acid, ononin, calycosin-7-O-b-D-glucoside, and panaxytriol might have negative effects on RBC aggregation, RBC deformability, intrinsic clotting activity, and platelet aggregation. Both CHFs and organisms are very complex systems and the specific interactions between them are still unclear; therefore, further exploration and verification are needed to understand the pharmacological mechanisms of the 4 components listed above. Nevertheless, this study demonstrated that one specific aspect of the vascular MK-2206 Akt inhibitor system might be affected by two or more CXC components, and the same component might have a positive effect on one aspect yet a negative effect on another, which reflects both the synergistic and the antagonistic actions of CHF components. Comprehensive qualitative or quantitative detection based on the low or high content of core bioactive components is necessary to improve CXC quality control. Therefore, we established the bioactive HPLC fingerprint of CXC based on its core bioactive components. Avian eggs contain maternally-derived biologically active substances that have the potential to influence developmental programmes of the next generation. Through such transgenerational effects, the phenotypic variability of the progeny can be manipulated to promote rapid adaptations to prevailing environmental conditions. Yolk sex steroids, mainly androgens, represent the most powerful agents that have been thoroughly studied by in ovo injection of exogenous hormones prior to incubation. Numerous studies have shown that yolk androgen transfer into the egg is a flexible process, varying with different social and environmental conditions. However, nearly the same amount of phenotypic variability of yolk testosterone concentrations is explained by genetic differences among females, as was experimentally demonstrated by divergent selection for yolk T concentrations in Japanese quail and also by correlative studies on similarities between mothers and daughters in small passerine birds, collared flycatchers and canaries. Thus, yolk androgen-mediated maternal effects can be described as indirect genetic effects with emerging evolutionary implications. Experimental studies demonstrated that increased yolk androgen levels influence immune functions in offspring during the early as well as later stages after hatching, but both reduced and enhanced immune responsiveness have been found. Concerning other variables, the immune-modulating effects of yolk T varied in the dose-dependent manner, ontogenetic development and the type of immune response.